| First Author | Shin HS | Year | 1982 |
| Journal | Cell | Volume | 29 |
| Issue | 3 | Pages | 969-76 |
| PubMed ID | 6295638 | Mgi Jnum | J:6934 |
| Mgi Id | MGI:55405 | Doi | 10.1016/0092-8674(82)90460-3 |
| Citation | Shin HS, et al. (1982) Genetic structure and origin of t haplotypes of mice, analyzed with H-2 cDNA probes. Cell 29(3):969-76 |
| abstractText | We investigated the genetic organization and evolutionary origin of t chromosomes of mice by examining the restriction fragment patterns of DNA from t haplotypes and normal chromosomes with cDNA probes to H-2 class I genes. On genomic DNA blots, the restriction fragments containing H-2-related sequences were highly variable among different inbred strains of mice, whereas they were very similar among different t haplotypes even when the t haplotypes carried serologically different H-2 haplotypes. These observations suggest that all t haplotypes have a common origin and are not products of independent mutational events. We also mapped the position of several restriction fragments characteristic of t DNA by using a battery of recombinant t haplotypes, defined with respect to their t-lethal factors and H-2 haplotypes. We thus show that restriction fragments containing H-2-related sequences map to the left of the H-2 class I genes in t chromosomes, a region in which the tw32 b-lethal factor also maps. The cloning of these fragments can be expected to provide an entry for the structural analysis of t DNA. |
