| First Author | Hams E | Year | 2020 |
| Journal | Front Immunol | Volume | 11 |
| Pages | 1966 | PubMed ID | 32973801 |
| Mgi Jnum | J:301996 | Mgi Id | MGI:6506285 |
| Doi | 10.3389/fimmu.2020.01966 | Citation | Hams E, et al. (2020) Role for Retinoic Acid-Related Orphan Receptor Alpha (RORalpha) Expressing Macrophages in Diet-Induced Obesity. Front Immunol 11:1966 |
| abstractText | The transcription factor RORalpha plays an important role in regulating circadian rhythm, inflammation, metabolism, and cellular development. Herein we show a role for RORalpha-expressing macrophages in the adipose tissue in altering the metabolic state of mice on a high-fat diet. The expression of Rora and RORA is elevated in white adipose tissue from obese mice and humans when compared to lean counterparts. When fed a high-fat diet Rora reporter mice revealed increased expression of Rora-YFP in macrophages in white adipose tissue deposits. To further define the potential role for Rora-expressing macrophages in the generation of an aberrant metabolic state Rora (fl/fl)LysM(Cre/+) mice, which do not express Rora in myeloid cells, were maintained on a high-fat diet, and metabolic parameters assessed. These mice had significantly impaired weight gain and improved metabolic parameters in comparison to Rora (fl/fl) control mice. Further analysis of the immune cell populations within white adipose tissue deposits demonstrates a decrease in inflammatory adipose tissue macrophages (ATM). In obese reporter mouse there was increased in Rora-YFP expressing ATM in adipose tissue. Analysis of peritoneal macrophage populations demonstrates that within the peritoneal cavity Rora-expression is limited to myeloid-derived macrophages, suggesting a novel role for RORalpha in macrophage development and activation, which can impact on metabolism, and inflammation. |
