| First Author | McNeish JD | Year | 1988 |
| Journal | Science | Volume | 241 |
| Issue | 4867 | Pages | 837-9 |
| PubMed ID | 3406741 | Mgi Jnum | J:9298 |
| Mgi Id | MGI:57760 | Doi | 10.1126/science.3406741 |
| Citation | McNeish JD, et al. (1988) Legless, a novel mutation found in PHT1-1 transgenic mice. Science 241(4867):837-9 |
| abstractText | In this report it is shown that the PHT1-1 line of transgenic mice exhibited a pattern of developmental abnormalities when the mice were homozygous for the transgene insertion. Hindlimbs were uniformly truncated at the distal end of the femur, resulting in a legless appearance. Forelimbs lacked anterior structures including digits and the radius. The brains had many defects, particularly in the anterior structures of the cerebrum, including the olfactory lobes. Craniofacial malformations in the form of facial clefts also commonly occurred. Furthermore, heterozygotes of this line, with only one copy of the DNA insertion, and other transgenic lines carrying the same DNA construct appeared normal, suggesting that in the PHT1-1 line a gene significant in mammalian development has been disrupted. |
