| First Author | Bell SM | Year | 1998 |
| Journal | Mech Dev | Volume | 74 |
| Issue | 1-2 | Pages | 41-50 |
| PubMed ID | 9651475 | Mgi Jnum | J:49210 |
| Mgi Id | MGI:1276999 | Doi | 10.1016/s0925-4773(98)00065-3 |
| Citation | Bell SM, et al. (1998) The loss of ventral ectoderm identity correlates with the inability to form an AER in the legless hindlimb bud. Mech Dev 74(1-2):41-50 |
| abstractText | We have characterized the early stages of murine hindlimb morphogenesis in the legless (lgl)mutant and non-mutant littermates. Initially the entire ventral ectoderm expresses many genetic markers characteristic of the AER (en-1, fgf-8, msx-2, dlx-2, cd44, and cx-43). Subsequently, the expression domain of most of these genes is restricted to the thickened ectoderm of the disto-ventral limb margin prior to forming an AER. In lgl, the expression of these genes is initiated but not maintained and the disto-ventral marginal ectoderm does not thicken. In contrast, Wnt7a expression is initiated and maintained in the dorsal ectoderm. The limb mesenchyme of lgl and non-mutant embyros initially expresses lmx-1b and fgf-10 uniformly. As the ventro-distal marginal ectoderm thickens, lmx-1b is progressively dorsally restricted in non-mutants but continues to be expressed ventrally in lgl hindlimb buds. These data suggest that establishment of a dorso-ventral ectodermal interface is not sufficient for AER formation and that restriction of lmx-1b to the dorsal mesenchyme is coordinately linked to AER formation. Copyright 1998 Elsevier Science Ireland Ltd. All rights reserved |
