| First Author | Chennupati V | Year | 2010 |
| Journal | J Immunol | Volume | 185 |
| Issue | 9 | Pages | 5160-8 |
| PubMed ID | 20870939 | Mgi Jnum | J:165201 |
| Mgi Id | MGI:4836438 | Doi | 10.4049/jimmunol.1001652 |
| Citation | Chennupati V, et al. (2010) Intra- and intercompartmental movement of gammadelta T cells: intestinal intraepithelial and peripheral gammadelta T cells represent exclusive nonoverlapping populations with distinct migration characteristics. J Immunol 185(9):5160-8 |
| abstractText | Unlike the approximately 1% of gammadelta TCR-positive T cells being regularly present in blood and secondary lymphoid organs (peripheral gammadelta T cells), approximately 50-60% of small intestinal intraepithelial lymphocytes (iIELs) in the mouse express the gammadelta TCR (gammadelta iIELs). In this study, we investigated the overlap and exchange of gammadelta iIELs and gammadelta T cells found in peripheral secondary lymphoid organs. Using two-photon laser-scanning microscopy, we found gammadelta T cells within peripheral lymph nodes to be highly motile, whereas gammadelta iIELs were characterized by a locally confined scanning behavior. Our results implied a strict separation of peripheral gammadelta T cells and gammadelta iIELs. Nevertheless, gammadelta iIELs could be efficiently regenerated from bone marrow-derived precursors in irradiated or T cell-deficient adult mice. However, outside the intestinal epithelium, survival of gammadelta iIELs was very poor. In CCR9-deficient mice, homing of gammadelta iIELs was impaired, but did not lead to an accumulation of gammadelta iIEL-like cells in the periphery. Conversely, in situations in which specific gammadelta iIEL niches were empty, adoptive transfer of isolated gammadelta iIELs led to a sustained engraftment of transferred gammadelta iIELs in the intestinal epithelium for at least 100 d. Furthermore, we demonstrated by heterotopic intestinal transplantation experiments that an exchange of gammadelta iIELs only rarely happens in the steady state of adult mice. We therefore conclude that peripheral versus intestinal intraepithelial gammadelta T cells are exclusive, nonoverlapping populations that virtually do not exchange with each other. |
