| First Author | Liu Z | Year | 2020 |
| Journal | Cell | Volume | 183 |
| Issue | 4 | Pages | 1117-1133.e19 |
| PubMed ID | 33096019 | Mgi Jnum | J:348716 |
| Mgi Id | MGI:6478335 | Doi | 10.1016/j.cell.2020.09.048 |
| Citation | Liu Z, et al. (2020) Detecting Tumor Antigen-Specific T Cells via Interaction-Dependent Fucosyl-Biotinylation. Cell 183(4):1117-1133.e19 |
| abstractText | Re-activation and clonal expansion of tumor-specific antigen (TSA)-reactive T cells are critical to the success of checkpoint blockade and adoptive transfer of tumor-infiltrating lymphocyte (TIL)-based therapies. There are no reliable markers to specifically identify the repertoire of TSA-reactive T cells due to their heterogeneous composition. We introduce FucoID as a general platform to detect endogenous antigen-specific T cells for studying their biology. Through this interaction-dependent labeling approach, intratumoral TSA-reactive CD4(+), CD8(+) T cells, and TSA-suppressive CD4(+) T cells can be detected and separated from bystander T cells based on their cell-surface enzymatic fucosyl-biotinylation. Compared to bystander TILs, TSA-reactive TILs possess a distinct T cell receptor (TCR) repertoire and unique gene features. Although exhibiting a dysfunctional phenotype, TSA-reactive CD8(+) TILs possess substantial capabilities of proliferation and tumor-specific killing. Featuring genetic manipulation-free procedures and a quick turnover cycle, FucoID should have the potential of accelerating the pace of personalized cancer treatment. |
