| First Author | Huang X | Year | 2020 |
| Journal | J Immunol | Volume | 205 |
| Issue | 7 | Pages | 1944-1952 |
| PubMed ID | 32859726 | Mgi Jnum | J:301515 |
| Mgi Id | MGI:6502434 | Doi | 10.4049/jimmunol.1900766 |
| Citation | Huang X, et al. (2020) IL-21 Promotes Intestinal Memory IgA Responses. J Immunol 205(7):1944-1952 |
| abstractText | The role of IL-21, produced mainly by Th17 cells and T follicular helper cells, has been intensively investigated in B cell differentiation and Ab class switch. However, how IL-21 regulates memory IgA(+) B cell development and memory IgA responses in the intestines is still not completely understood. In this study, we found the total IgA(+) B cells as well as CD38(+)CD138(-)IgA(+) memory B cells were significantly increased in intestinal lamina propria (LP) of TCRbetaxdelta(-/-) mice after transfer of microbiota Ag-specific Th17 cells but not Th1 cells. Although IL-21R(-/-) mice or IL-17R(-/-) mice showed decreased Ag-specific memory IgA production in the intestines upon infection with Citrobacter rodentium, the percentage of IgA(+)CD38(+)CD138(-) memory B cells in Peyer's patches and LP was decreased only in IL-21R(-/-) mice, but not in IL-17R(-/-) mice, after reinfection with C. rodentium compared with wild-type mice. Blockade IL-21 in vivo suppressed intestinal C. rodentium-specific IgA production as well as IgA(+)CD38(+)CD138(-) memory B cells in Peyer's patches and LP. Furthermore, IL-21 significantly induced B cell IgA production in vitro, with the increased expression of genes related with class-switching and memory B cell development, including Aicda, Ski, Bmi1, and Klf2. Consistently, Aicda and Ski expression was decreased in B cells of IL-21R(-/-) mice after C. rodentium reinfection. In conclusion, our study demonstrated that IL-21 promotes intestinal memory IgA B cell development, possibly through upregulating differentiation-related and class switching-related genes, indicating a potential role of IL-21 in memory IgA(+) B cell responses in the intestines. |
