| First Author | Bosco N | Year | 2008 |
| Journal | Eur J Immunol | Volume | 38 |
| Issue | 12 | Pages | 3520-9 |
| PubMed ID | 18991270 | Mgi Jnum | J:141408 |
| Mgi Id | MGI:3818222 | Doi | 10.1002/eji.200838668 |
| Citation | Bosco N, et al. (2008) TCR-beta chains derived from peripheral gammadelta T cells can take part in alphabeta T-cell development. Eur J Immunol 38(12):3520-3529 |
| abstractText | Between 10 and 20% of the peripheral gammadelta T cells express cytoplasmic TCR-beta proteins, but whether such TCR-beta chains can partake in alphabeta T-cell development has never been systematically investigated. Therefore, we reconstituted the T-cell compartment of CD3epsilon-deficient mice with Pax5-TCR-beta deficient proB cells expressing, via a retroviral vector, TCR-beta chains from either peripheral gammadelta or alphabeta T cells. Recipient thymi reconstituted with proB cells containing empty vector were small (<15x10(6) cells), contained few gammadelta T but no alphabeta T cells. In contrast, thymi from mice receiving proB cells containing gammadelta or alphabeta T-cell-derived TCR-beta chains contained 80-130x10(6) cells, and showed a normal CD4, CD8 and alphabeta TCR expression pattern. However, regardless of the source of TCR-beta chain, reconstituted mice rapidly showed signs of autoimmunity dying 5-15 wk following reconstitution. Autoimmune disease induction could be prevented by co-transfer of Treg cells thereby allowing the functionality of the generated T cells to be assessed. Results obtained show that TCR-beta chains from gammadelta T cells can efficiently take part in alphabeta T-cell development. The implications of these findings for gammadelta T-cell development will be discussed. |
