| First Author | O'Brien RL | Year | 2012 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 53 |
| Issue | 1 | Pages | 301-8 |
| PubMed ID | 22199243 | Mgi Jnum | J:191499 |
| Mgi Id | MGI:5461980 | Doi | 10.1167/iovs.11-8855 |
| Citation | O'Brien RL, et al. (2012) alphabeta TCR(+) T cells, but not B cells, promote autoimmune keratitis in b10 mice lacking gammadelta T cells. Invest Ophthalmol Vis Sci 53(1):301-8 |
| abstractText | PURPOSE: To investigate additional factors in the spontaneous development of keratitis previously reported in B10.TCRdelta(-)/(-) female mice. METHODS: The study tested whether susceptible B10.TCRdelta(-)/(-) mice have dry eyes compared with resistant B6.TCRdelta(-)/(-) females and also rederived the B10.TCRdelta(-)/(-) strain to test for the role of an infectious agent. Also assessed was whether adoptive transfer of alphabeta T cells from autoimmune mice induced keratitis in resistant mice. In addition, a potential role was examined for B cells or autoantibodies by B-cell inactivation, and the role of female hormones was tested by ovariectomy. Finally, the study investigated whether adoptive transfer of Vgamma1(+) gammadelta T cells confers protection. RESULTS: Tear production in B10.TCRdelta(-)/(-) females was actually higher than in B6.TCRdelta(-)/(-) controls. Rederived B10.TCRdelta(-)/(-) mice still developed keratitis. Keratitis was induced in resistant mice after adoptive transfer of alphabeta T cells from keratitic donors. Inactivation of B cells from susceptible mice had no effect on the development of keratitis. Ovariectomy did not significantly reduce disease in B10.TCRdelta(-)/(-) females. Adoptive transfer of Vgamma1(+) cells from wild-type donors reduced keratitis in B10.TCRdelta(-)/(-) females. CONCLUSIONS: Neither low tear levels nor ovarian hormones contribute to spontaneous keratitis in B10.TCRdelta(-)/(-) female mice, nor does it appear to depend on an infectious agent carried vertically in this strain. However, alphabeta T cells from keratitic hosts are sufficient to induce disease in the resistant B10.TCRbeta(-)/(-)delta(-)/(-) strain. Autoaggressive alphabeta T cells in the absence of Vgamma1(+) T cells in B10.TCRdelta(-)/(-) mice may be insufficiently checked to prevent disease. |
