| First Author | Joubert IA | Year | 2020 |
| Journal | Vaccine | Volume | 38 |
| Issue | 5 | Pages | 1015-1024 |
| PubMed ID | 31812465 | Mgi Jnum | J:287015 |
| Mgi Id | MGI:6405839 | Doi | 10.1016/j.vaccine.2019.11.051 |
| Citation | Joubert IA, et al. (2020) Mast cells and gammadelta T cells are largely dispensable for adaptive immune responses after laser-mediated epicutaneous immunization. Vaccine 38(5):1015-1024 |
| abstractText | BACKGROUND: The skin resembles an attractive target for vaccination due to its accessibility and abundance of resident immune cells. Cells like gammadelta T cells and mast cells (MCs) are part of the first line of defence against exogenous threats. Despite being important mediators for eliciting TH2 immune responses after epithelial stress, gammadelta T cell and MC functions still remain to be completely understood. Here, we aimed to characterize their roles in shaping adaptive immune responses after laser-mediated epicutaneous immunization (EPI). METHODS: gammadelta T cell knock out, MC-depleted, and wildtype control mice were immunized with mannan-conjugated grass pollen allergen Phl p 5 (P5-MN) by laser-mediated EPI. After 2-3 immunizations, cytokine expression, T helper polarization, and antigen-specific IgG1/IgE levels were analysed. Furthermore, the local cytokine/chemokine milieu after laser microporation was determined. RESULTS: The majority of inflammatory chemokines and cytokines induced by laser treatment were not affected by the presence of gammadelta T cells or MCs. However, RANTES was elevated in gammadelta T cell knock out mice and GROalpha, TSLP, and IL-33 were significantly decreased after MC depletion. The absence of gammadelta T cells or depletion of MCs had no substantial effect on adaptive immune responses after laser-mediated EPI, except for slightly reduced IgG1 and effector T cell levels in MC-depleted mice. CONCLUSIONS: gammadelta T cells did not play a pivotal role in shaping the humoral and cellular adaptive immune response after laser-mediated EPI. MC depletion decreased the numbers of effector T cells, indicating a potential role of MCs in the activation and maturation of T cells after EPI. |
