| First Author | Walker CR | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 12 | Pages | e84553 |
| PubMed ID | 24358364 | Mgi Jnum | J:209854 |
| Mgi Id | MGI:5568825 | Doi | 10.1371/journal.pone.0084553 |
| Citation | Walker CR, et al. (2013) Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge. PLoS One 8(12):e84553 |
| abstractText | Antimicrobial proteins influence intestinal microbial ecology and limit proliferation of pathogens, yet the regulation of their expression has only been partially elucidated. Here, we have identified a putative pathway involving epithelial cells and intestinal intraepithelial lymphocytes (iIELs) that leads to antimicrobial protein (AMP) production by Paneth cells. Mice lacking gammadelta iIELs (TCRdelta(-/-)) express significantly reduced levels of the AMP angiogenin 4 (Ang4). These mice were also unable to up-regulate Ang4 production following oral challenge by Salmonella, leading to higher levels of mucosal invasion compared to their wild type counterparts during the first 2 hours post-challenge. The transfer of gammadelta iIELs from wild type (WT) mice to TCRdelta(-/-) mice restored Ang4 production and Salmonella invasion levels were reduced to those obtained in WT mice. The ability to restore Ang4 production in TCRdelta(-/-) mice was shown to be restricted to gammadelta iIELs expressing Vgamma7-encoded TCRs. Using a novel intestinal crypt co-culture system we identified a putative pathway of Ang4 production initiated by exposure to Salmonella, intestinal commensals or microbial antigens that induced intestinal epithelial cells to produce cytokines including IL23 in a TLR-mediated manner. Exposure of TCR-Vgamma7(+) gammadelta iIELs to IL-23 promoted IL22 production, which triggered Paneth cells to secrete Ang4. These findings identify a novel role for gammadelta iIELs in mucosal defence through sensing immediate epithelial cell cytokine responses and influencing AMP production. This in turn can contribute to the maintenance of intestinal microbial homeostasis and epithelial barrier function, and limit pathogen invasion. |
