| First Author | Jin JZ | Year | 2014 |
| Journal | Dev Dyn | Volume | 243 |
| Issue | 12 | Pages | 1536-43 |
| PubMed ID | 25104574 | Mgi Jnum | J:216310 |
| Mgi Id | MGI:5608629 | Doi | 10.1002/dvdy.24177 |
| Citation | Jin JZ, et al. (2014) Deciphering TGF-beta3 function in medial edge epithelium specification and fusion during mouse secondary palate development. Dev Dyn 243(12):1536-43 |
| abstractText | BACKGROUND: Transforming growth factor-beta3 (TGF-beta3) plays a central role in mediating secondary palate fusion along the facial midline. However, the mechanisms by which TGF-beta3 functions during secondary palate fusion are still poorly understood. RESULTS: We found that mouse cytokeratin 6alpha and 17 mRNAs were expressed exclusively in the palate medial edge epithelium on embryonic day 14.5, and this expression was completely abolished in Tgf-beta3 mutant embryos. In contrast, we found that Jagged2 was initially expressed throughout the palate epithelium, but was specifically down-regulated in the medial edge epithelium during palatal fusion. Jagged2 down-regulation was regulated by TGF-beta3, since Jagged2 was persistently expressed in palatal medial edge epithelium in Tgf-beta3 null mutant embryos. Moreover, addition of DAPT, a specific inhibitor of Notch signaling, partially rescued the fusion defects in Tgf-beta3 null mutant palatal shelves. CONCLUSIONS: Based on these results, together with the previous study indicating that the loss of Jagged2 function promotes embryonic oral epithelial fusion, we concluded that TGF-beta3 mediates palate fusion in part by down-regulating Jagged2 expression in palatal medial edge epithelium. In addition, cytokeratin 6alpha and 17 are two TGF-beta3 downstream target genes in palate medial edge epithelium differentiation. Developmental Dynamics 243:1536-1543, 2014. (c) 2014 Wiley Periodicals, Inc. |
