| First Author | Zhou J | Year | 2020 |
| Journal | Biochem Biophys Res Commun | Volume | 529 |
| Issue | 4 | Pages | 1165-1172 |
| PubMed ID | 32819581 | Mgi Jnum | J:302361 |
| Mgi Id | MGI:6508208 | Doi | 10.1016/j.bbrc.2020.06.140 |
| Citation | Zhou J, et al. (2020) Bmi-1 determines the stemness of renal stem or progenitor cells. Biochem Biophys Res Commun 529(4):1165-1172 |
| abstractText | Renal stem or progenitor cells (RSCs), labeled with CD24 and CD133, play an important role during the repair of renal injury. Bmi-1 is a critical factor in regulating stemness of adult stem cells or progenitor cells. To investigate whether Bmi-1 determines the stemness of RSCs by inhibiting p16 and p53, and/or maintaining redox balance, RSCs were isolated, cultured and analyzed for stemness characterizations. In RSCs from Bmi-1-deficient (Bmi-1(-/-)) mice and wild type (WT) littermates, self-renewal, stemness, and expressions of molecules for regulating redox balance and cell cycle progression were compared. Self-renewal of RSCs from Bmi-1 and p16 double-knockout (Bmi-1(-/-)p16(-/-)), Bmi-1 and p53 double-knockout (Bmi-1(-/-)p53(-/-)) and N-acetylcysteine (NAC)-treated Bmi-1(-/-) mice were further analyzed for amelioration. Human renal proximal tubular epithelial cells (HK2) were also used for signaling analysis. Our results showed that third-passage RSCs from WT mice had good stemness; Bmi-1 deficiency led to the decreased stemness, and the increased apoptosis for RSCs; NAC treatment or p16/p53 deletion ameliorated the decreased self-renewal of RSCs in Bmi-1 deficiency mice by maintaining redox balance or inhibiting cell cycle arrest respectively; Oxidative stress (OS) could negatively feedback regulate the mRNA expressions of Bmi-1, p16 and p53. In conclusion, Bmi-1 determined the stemness of RSCs through maintaining redox balance and preventing cell cycle arrest. Thus, Bmi-1 signaling molecules would be novel therapeutic targets for maintaining RSCs and hampering the progression of kidney diseases to prevent renal failure. |
