| First Author | Singh I | Year | 2013 |
| Journal | Proc Natl Acad Sci U S A | Volume | 110 |
| Issue | 36 | Pages | 14771-6 |
| PubMed ID | 23959870 | Mgi Jnum | J:200976 |
| Mgi Id | MGI:5510601 | Doi | 10.1073/pnas.1302212110 |
| Citation | Singh I, et al. (2013) Low levels of copper disrupt brain amyloid-beta homeostasis by altering its production and clearance. Proc Natl Acad Sci U S A 110(36):14771-6 |
| abstractText | Whereas amyloid-beta (Abeta) accumulates in the brain of normal animals dosed with low levels of copper (Cu), the mechanism is not completely known. Cu could contribute to Abeta accumulation by altering its clearance and/or its production. Because Cu homeostasis is altered in transgenic mice overexpressing Abeta precursor protein (APP), the objective of this study was to elucidate the mechanism of Cu-induced Abeta accumulation in brains of normal mice and then to explore Cu's effects in a mouse model of Alzheimer's disease. In aging mice, accumulation of Cu in brain capillaries was associated with its reduction in low-density lipoprotein receptor-related protein 1 (LRP1), an Abeta transporter, and higher brain Abeta levels. These effects were reproduced by chronic dosing with low levels of Cu via drinking water without changes in Abeta synthesis or degradation. In human brain endothelial cells, Cu, at its normal labile levels, caused LRP1-specific down-regulation by inducing its nitrotyrosination and subsequent proteosomal-dependent degradation due in part to Cu/cellular prion protein/LRP1 interaction. In APP(sw/0) mice, Cu not only down-regulated LRP1 in brain capillaries but also increased Abeta production and neuroinflammation because Cu accumulated in brain capillaries and, unlike in control mice, in the parenchyma. Thus, we have demonstrated that Cu's effect on brain Abeta homeostasis depends on whether it is accumulated in the capillaries or in the parenchyma. These findings should provide unique insights into preventative and/or therapeutic approaches to control neurotoxic Abeta levels in the aging brain. |
