| First Author | Sciesielski LK | Year | 2010 |
| Journal | FEBS Lett | Volume | 584 |
| Issue | 22 | Pages | 4665-71 |
| PubMed ID | 20974136 | Mgi Jnum | J:167560 |
| Mgi Id | MGI:4868543 | Doi | 10.1016/j.febslet.2010.10.045 |
| Citation | Sciesielski LK, et al. (2010) Wilms' tumor protein Wt1 regulates the Interleukin-10 (IL-10) gene. FEBS Lett 584(22):4665-71 |
| abstractText | We identified the Wilms' tumor protein, Wt1, as a novel transcriptional activator of the immunosuppressant cytokine interleukin-10 (IL-10). Silencing of Wt1 by RNA interference reduced IL-10 mRNA levels by approximately 90%. IL-10 transcripts were increased more than 15-fold upon forced expression of Wt1. Electrophoretic mobility shift assay and chromatin immunoprecipitation revealed a cis-element that was responsible for activation of the IL-10 promoter by Wt1 in murine macrophages. Mutation of the Wt1 binding motif abrogated stimulation of the IL-10 promoter by tumor necrosis factor-alpha (TNFalpha). These results suggest a novel immune regulatory function of Wt1 in controlling IL-10 gene expression. |
