| First Author | Osawa N | Year | 1992 |
| Journal | Mouse Genome | Volume | 90 |
| Issue | 2 | Pages | 223 |
| Mgi Jnum | J:1273 | Mgi Id | MGI:49803 |
| Citation | Osawa N, et al. (1992) New neurological mutant "wriggle mouse sagami" with an interesting movement disorder. Mouse Genome 90(2):223 |
| abstractText | Full text of Mouse Genome contribution: New Neurological Mutant "Wriggle Mouse Sagami" With An Interesting Movement Disorder. Nobutaka Osawa and Michio Ohmura*. Department of Microbiology, Kitasato University School of Medicine, Sagamihara 228, JAPAN. *Ohmura Institute for Laboratory Animals, Zama 228, JAPAN. In 1984, a mutant with a hereditary neurological disorder, characterized by various kinds of abnormal movement on awakening, was found in inbred strain BALB/cAnN mice at the Ohmura Institute for Laboratory Animals (1). Although the mutant shows no sign of disease at the time of birth, initial clinical signs begin to appear about 10 days after birth and rapidly become evident. The mutant can neither perform the normal gait nor maintain upright posture because of its abnormal movement consisting of tremor and choreo-athetosis movement associated with the dystonic posture. Concerning the mutation, it was confirmed that the syndrome was due to an autosomal recessive gene. We successfully bred the mouse separately as a mutant strain and named it "Wriggle Mouse Sagami (WMS)" because of the syndrome. The gene symbol was designated as "wri". Genetic studies. Results of progeny tests provided evidence of inheritance of the syndrome as a single autosomal recessive gene. Summarized results are shown in Tables la and 1b. Results of linkage tests with coat colour loci using BALB/c, C57BL/6, DBA/2, C57L, and nude mice, were assigned to the following chromosomes, showed no evidence of close linkage: ln (Chr 1), A (Chr 2), b (Chr 4), c (Chr 7), and d (Chr 9 ). In crosses between doubly heterozygous animals of genotype wri+/+nu 85 mice were born. Of these 15 were affected, and all of them had normal coats. Eighteen nude mice did not show the abnormality. However, a X2 test for linkage between wri and nu (Chr 11) was not statistically significant (X2=2.89). Table 1a. Segregation of Wriggle Mouse Sagami observed in the BALB/c (Hetero) line. Mutant line: 1; No. of matings: 19; normal: female: 49; male: 44; total: 93; affected: female: 28; male: 10; total: 38; ratio (n:a): 2.44:1. Mutant line: 2; No. of matings: 43; normal: female: 100; male: 90; total: 190; affected: female: 39; male: 28; total: 67; ratio (n:a): 2.83:1. Mutant line: 3; No. of matings: 25; normal: female: 51; male: 44; total: 95; affected: female: 25; male: 14; total: 39; ratio (n:a): 2.43:1. Totals: No. of matings: 87; normal: female: 200; male: 178; total: 378; affected: female: 92; male: 52; total: 144; ratio (n:a): 2.63:1. Table 1b. Segregation of affected mice among progeny from various types of mating. Matings: female: affected* (wri/wri); male: normal (+/+); No. of matings: 3; No. of progenies: 21; normal: female: 12; male: 9; total: 21. Matings: female: affected* (wri/wri); male: hetero (wri/+); No. of matings: 8; No. of progenies: 41; normal: female: 13; male: 8; total: 21; affected: female: 12; male: 8; total: 20. Matings: female: WMS (wri/wri); male: hetero (wri/+); No. of matings: 3; No. of progenies: 11; normal: female: 2; male: 3; total: 5; affected: female: 5; male: 1; total: 6. Matings: female: hetero (wri/+); male: hetero (wri/+); No. of matings: 87; No. of progenies: 522; normal: female: 200; male: 178; total: 378; affected: female: 92; male: 52; total: 144. *BALB/c X C3H F1 female with ovary transplanted from Wriggle Mouse Sagami. Coloured progeny was removed. |
