| First Author | Aspord C | Year | 2004 |
| Journal | J Autoimmun | Volume | 23 |
| Issue | 1 | Pages | 27-35 |
| PubMed ID | 15236750 | Mgi Jnum | J:91668 |
| Mgi Id | MGI:3050169 | Doi | 10.1016/j.jaut.2004.03.007 |
| Citation | Aspord C, et al. (2004) Early events in islets and pancreatic lymph nodes in autoimmune diabetes. J Autoimmun 23(1):27-35 |
| abstractText | The specific contributions of islet cell microenvironment during the development of autoimmune type 1 diabetes remain unclear. The aims of this study were to identify early immune-driven abnormalities in islets and pancreatic lymph nodes of NOD mice by cDNA arrays. We compared gene expression profiles of purified islets and pancreatic lymph nodes of 4-week-old NOD mice to NOD-SCID and BALB/c mice. To further characterize the networks implicated in beta-cell destruction, we also performed a time-course analysis using islets and pancreatic lymph nodes of NOD mice from 2 to 25 weeks of age. We found consistent changes by cDNA arrays and RT-PCR analyses among islet genes before the detection of CD3+ T cells in the islet periphery associated with dendritic cell attraction, lymphocyte homing, and apoptosis. In contrast to IL-1, TYNFSF13B and osteopontin genes which were specifically activated, the immunoregulatory cytokine IL-11 was poorly detected in NOD islets and pancreatic lymph nodes. Genes involved in angiogenesis were also specifically activated in NOD islets of 2 and 4 weeks of age. The present time-course macroarray and RT-PCR analyses provides a detailed picture of the different genes involved in autoimmune diabetes and illustrates the importance of islet cell microenvironment that prepares the late beta-cell destruction. |
