| First Author | Ford ML | Year | 2006 |
| Journal | Cell Immunol | Volume | 240 |
| Issue | 1 | Pages | 53-61 |
| PubMed ID | 16890924 | Mgi Jnum | J:112761 |
| Mgi Id | MGI:3663532 | Doi | 10.1016/j.cellimm.2006.06.007 |
| Citation | Ford ML, et al. (2006) Modulation of MOG 37-50-specific CD8+ T cell activation and expansion by CD43. Cell Immunol 240(1):53-61 |
| abstractText | Several recent reports have described an effector role for CD8(+) T cells during EAE. We have previously demonstrated reduced disease incidence and severity in CD43(-/-) mice following MOG immunization, and attributed this attenuation in disease progression to the effects of CD43 deficiency on CD4+ T cells. Here, we extend those studies to examine the effects of the loss of CD43 on MOG-specific CD8+ T cells. A reduced frequency of MOG-specific CD8+ T cells following immunization was observed in CD43(-/-) mice relative to wild-type controls, as demonstrated by intracellular cytokine and MHC tetramer staining. In addition, adoptive transfer of CD8+ MOG 35-55-primed LN cells from CD43(-/-) mice resulted in significantly attenuated EAE induction as compared to recipients of wild-type CD8+ MOG-primed cells. Analysis of intracellular signaling intermediates revealed a deficiency in the ability of MOG-specific CD8+ T cells to phosphorylate ERK in response to antigen. These results characterize an important role for CD43 during the activation and expansion of autoreactive MOG-specific CD8+ T cells. |
