| First Author | Ye W | Year | 2015 |
| Journal | J Hematol Oncol | Volume | 8 |
| Pages | 59 | PubMed ID | 26022250 |
| Mgi Jnum | J:327216 | Mgi Id | MGI:7329010 |
| Doi | 10.1186/s13045-015-0156-y | Citation | Ye W, et al. (2015) Quantitative evaluation of the immunodeficiency of a mouse strain by tumor engraftments. J Hematol Oncol 8(59) |
| abstractText | Background: The mouse is an organism that is widely used as a mammalian model for studying human physiology or disease, and the development of immunodeficient mice has provided a valuable tool for basic and applied human disease research. Following the development of large-scale mouse knockout programs and genome-editing tools, it has become increasingly efficient to generate genetically modified mouse strains with immunodeficiency. However, due to the lack of a standardized system for evaluating the immuno-capacity that prevents tumor progression in mice, an objective choice of the appropriate immunodeficient mouse strains to be used for tumor engrafting experiments is difficult. Methods: In this study, we developed a tumor engraftment index (TEI) to quantify the immunodeficiency response to hematologic malignant cells and solid tumor cells of six immunodeficient mouse strains and C57BL/6 wild-type mouse (WT). Results: Mice with a more severely impaired immune system attained a higher TEI score. We then validated that the NOD-scid-IL2Rg-/- (NSI) mice, which had the highest TEI score, were more suitable for xenograft and allograft experiments using multiple functional assays. Conclusions: The TEI score was effectively able to reflect the immunodeficiency of a mouse strain. |
