| First Author | Agarwal Y | Year | 2020 |
| Journal | Sci Rep | Volume | 10 |
| Issue | 1 | Pages | 14598 |
| PubMed ID | 32884084 | Mgi Jnum | J:296370 |
| Mgi Id | MGI:6467153 | Doi | 10.1038/s41598-020-71548-z |
| Citation | Agarwal Y, et al. (2020) Development of humanized mouse and rat models with full-thickness human skin and autologous immune cells. Sci Rep 10(1):14598 |
| abstractText | The human skin is a significant barrier for protection against pathogen transmission. Rodent models used to investigate human-specific pathogens that target the skin are generated by introducing human skin grafts to immunocompromised rodent strains. Infection-induced immunopathogenesis has been separately studied in humanized rodent models developed with human lymphoid tissue and hematopoietic stem cell transplants. Successful co-engraftment of human skin, autologous lymphoid tissues, and autologous immune cells in a rodent model has not yet been achieved, though it could provide a means of studying the human immune response to infection in the human skin. Here, we introduce the human Skin and Immune System (hSIS)-humanized NOD-scid IL2Rgamma(null) (NSG) mouse and Sprague-Dawley-Rag2(tm2hera) Il2rgamma(tm1hera) (SRG) rat models, co-engrafted with human full-thickness fetal skin, autologous fetal lymphoid tissues, and autologous fetal liver-derived hematopoietic stem cells. hSIS-humanized rodents demonstrate the development of human full-thickness skin, along with autologous lymphoid tissues, and autologous immune cells. These models also support human skin infection following intradermal inoculation with community-associated methicillin-resistant Staphylococcus aureus. The co-engraftment of these human skin and immune system components into a single humanized rodent model could provide a platform for studying human skin infections. |
