| First Author | Lin Y | Year | 2023 |
| Journal | JCI Insight | Volume | 8 |
| Issue | 5 | PubMed ID | 36692963 |
| Mgi Jnum | J:334925 | Mgi Id | MGI:7460680 |
| Doi | 10.1172/jci.insight.164781 | Citation | Lin Y, et al. (2023) Origin, prospective identification, and function of circulating endothelial colony-forming cells in mice and humans. JCI Insight 8(5) |
| abstractText | Most circulating endothelial cells are apoptotic, but rare circulating endothelial colony-forming cells (C-ECFCs), also known as blood outgrowth endothelial cells, with proliferative and vasculogenic activity can be cultured; however, the origin and naive function of these C-ECFCs remains obscure. Herein, detailed lineage tracing revealed murine C-ECFCs emerged in the early postnatal period, displayed high vasculogenic potential with enriched frequency of clonal proliferative cells compared with tissue-resident ECFCs, and were not committed to or derived from the BM hematopoietic system but from tissue-resident ECFCs. In humans, C-ECFCs were present in the CD34bright cord blood mononuclear subset, possessed proliferative potential and in vivo vasculogenic function in a naive or cultured state, and displayed a single cell transcriptome sharing some umbilical venous endothelial cell features, such as a higher protein C receptor and extracellular matrix gene expression. This study provides an advance for the field by identifying the origin, naive function, and antigens to prospectively isolate C-ECFCs for translational studies. |
