| First Author | Secatto A | Year | 2012 |
| Journal | PLoS One | Volume | 7 |
| Issue | 3 | Pages | e31701 |
| PubMed ID | 22448213 | Mgi Jnum | J:187034 |
| Mgi Id | MGI:5435142 | Doi | 10.1371/journal.pone.0031701 |
| Citation | Secatto A, et al. (2012) 5-Lipoxygenase deficiency impairs innate and adaptive immune responses during fungal infection. PLoS One 7(3):e31701 |
| abstractText | 5-Lipoxygenase-derived products have been implicated in both the inhibition and promotion of chronic infection. Here, we sought to investigate the roles of endogenous 5-lipoxygenase products and exogenous leukotrienes during Histoplasma capsulatum infection in vivo and in vitro. 5-LO deficiency led to increased lung CFU, decreased nitric oxide production and a deficient primary immune response during active fungal infection. Moreover, H. capsulatum-infected 5-LO(-/-) mice showed an intense influx of neutrophils and an impaired ability to generate and recruit effector T cells to the lung. The fungal susceptibility of 5-LO(-/-) mice correlated with a lower rate of macrophage ingestion of IgG-H. capsulatum relative to WT macrophages. Conversely, exogenous LTB4 and LTC4 restored macrophage phagocytosis in 5-LO deficient mice. Our results demonstrate that leukotrienes are required to control chronic fungal infection by amplifying both the innate and adaptive immune response during histoplasmosis. |
