| First Author | Iqbal J | Year | 2005 |
| Journal | J Lipid Res | Volume | 46 |
| Issue | 7 | Pages | 1491-501 |
| PubMed ID | 15834127 | Mgi Jnum | J:100497 |
| Mgi Id | MGI:3588631 | Doi | 10.1194/jlr.M500023-JLR200 |
| Citation | Iqbal J, et al. (2005) Evidence for multiple complementary pathways for efficient cholesterol absorption in mice. J Lipid Res 46(7):1491-501 |
| abstractText | Apolipoprotein B (apoB)-dependent and apoB-independent pathways for cholesterol transport have been described in cultured cells. Here, we show that the apoB-independent pathway involves apoA-I-containing high density lipoproteins (HDLs). Cholesterol secretion by the HDLs, but not by the apoB pathway, was significantly reduced in primary enterocytes isolated from chow- and cholesterol-fed apoA-I(-/-) mice. These enterocytes were capable of cholesterol efflux when apoA-I was provided extracellularly. In apoA-I(-/-) mice, the absorption of a bolus of cholesterol was similar in control and apoA-I(-/-) mice fed chow or high-cholesterol diet. However, short-term studies revealed that cholesterol absorption was occurring over longer lengths of the intestine, and cholesterol but not triglyceride transport to the plasma and liver in chow- and cholesterol-fed apoA-I(-/-) mice was significantly reduced. These studies indicate that in apoA-I deficiency, there is a delay in cholesterol absorption, but cholesterol is eventually absorbed because of the compensatory apoB pathway. Nonetheless, long-term studies involving multiple feedings showed significant reduction in cholesterol absorption after 4 days. We propose that multiple compensatory mechanisms ensure efficient cholesterol absorption in mice. |
