| First Author | Sauter M | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 3 | Pages | 101645 |
| PubMed ID | 36042879 | Mgi Jnum | J:361128 |
| Mgi Id | MGI:7850869 | Doi | 10.1016/j.xpro.2022.101645 |
| Citation | Sauter M, et al. (2022) Protocol to visualize CD11c(+) cells in atherosclerosis using LacZ reporter mice. STAR Protoc 3(3):101645 |
| abstractText | Here, we describe an in vivo approach to visualize CD11c(+) cells in atherosclerosis. In particular, we use a protocol for X-Gal staining of immune cells within atherosclerotic plaques, which can be used as an alternative to analyze plaque composition and cell-specific molecules in atherogenesis. LacZ knockin mice have to be bred to mice carrying the CD11c(cre) recombinase-both brought onto an ApoE(-/-) background-to be able to visualize this cell type of interest in the plaques by X-Gal staining. With this approach, different immune cells in atherogenesis can be examined. For complete details on the use and execution of this protocol, please refer to Sauter et al. (2021). |
