| First Author | Mendiratta SK | Year | 1999 |
| Journal | Int Immunol | Volume | 11 |
| Issue | 3 | Pages | 351-60 |
| PubMed ID | 10221647 | Mgi Jnum | J:110542 |
| Mgi Id | MGI:3640464 | Doi | 10.1093/intimm/11.3.351 |
| Citation | Mendiratta SK, et al. (1999) Peptide dependency of alloreactive CD4+ T cell responses. Int Immunol 11(3):351-60 |
| abstractText | Alloreactivity, the capacity of a large number of T lymphocytes to react with foreign MHC molecules, represents the cellular basis for the rejection of tissue grafts. Although it was originally assumed that the TCR of alloreactive T cells focus their recognition on the polymorphic residues that differ between the MHC molecules of responder and stimulator cells, studies in the MHC class I system have clearly demonstrated that MHC-bound peptides can influence this interaction. It remains unclear, however, whether peptides play an equally important role for the recognition of MHC class II molecules by alloreactive CD4+ T cells. Another issue that remains unresolved is the overall frequency of peptide-dependent versus peptide-independent alloreactive T cells. We have addressed these questions with antigen-presenting cells (APC) from H2-M mutant mice that predominantly express a single MHC class II-peptide complex, H2-Ab bound by a peptide (CLIP) derived from the class II-associated invariant chain. APC from these mice were used as targets and stimulators for alloreactive CD4+ T cells. Results demonstrated that the vast majority of CD4+ alloreactive T cells recognize MHC class II molecules in a peptide-dependent fashion. |
