| First Author | Köprülü AD | Year | 2013 |
| Journal | PLoS One | Volume | 8 |
| Issue | 3 | Pages | e60476 |
| PubMed ID | 23544144 | Mgi Jnum | J:199894 |
| Mgi Id | MGI:5505742 | Doi | 10.1371/journal.pone.0060476 |
| Citation | Koprulu AD, et al. (2013) The tyrosine kinase Btk regulates the macrophage response to Listeria monocytogenes infection. PLoS One 8(3):e60476 |
| abstractText | In this study we investigated the role of Bruton's tyrosine kinase (Btk) in the immune response to the Gram-positive intracellular bacterium Listeria monocytogenes (Lm). In response to Lm infection, Btk was activated in bone marrow-derived macrophages (BMMs) and Btk (-/-) BMMs showed enhanced TNF-alpha, IL-6 and IL-12p40 secretion, while type I interferons were produced at levels similar to wild-type (wt) BMMs. Although Btk-deficient BMMs displayed reduced phagocytosis of E. coli fragments, there was no difference between wt and Btk (-/-) BMMs in the uptake of Lm upon infection. Moreover, there was no difference in the response to heat-killed Lm between wt and Btk (-/-) BMMs, suggesting a role for Btk in signaling pathways that are induced by intracellular Lm. Finally, Btk (-/-) mice displayed enhanced resistance and an increased mean survival time upon Lm infection in comparison to wt mice. This correlated with elevated IFN-gamma and IL-12p70 serum levels in Btk (-/-) mice at day 1 after infection. Taken together, our data suggest an important regulatory role for Btk in macrophages during Lm infection. |
