| First Author | McCarrick JW 3rd | Year | 1993 |
| Journal | Transgenic Res | Volume | 2 |
| Issue | 4 | Pages | 183-90 |
| PubMed ID | 8364601 | Mgi Jnum | J:48775 |
| Mgi Id | MGI:1306669 | Doi | 10.1007/BF01977348 |
| Citation | McCarrick JW 3rd, et al. (1993) Positive-negative selection gene targeting with the diphtheria toxin A-chain gene in mouse embryonic stem cells. Transgenic Res 2(4):183-90 |
| abstractText | The diphtheria toxin A-chain gene was used in a positive-negative selection gene targeting vector to alter the CD4 gene which is transcriptionally silent in mouse embryonic stem cells. Expression of the toxin gene was driven by a constitutively active enhancer, yet the targeting construct exhibited only minimal transient toxicity while enriching for targeted clones 9- to 29-fold. Germline transmission of the stem cell-derived genome was obtained. These data suggest the usefulness of this diphtheria toxin A-chain cassette in replacement- type positive-negative selection vectors. Its potential for novel applications, particularly in the enrichment for 'hit-and-run' insertion-type vectors, is discussed. |
