| First Author | Abonia JP | Year | 2005 |
| Journal | Blood | Volume | 105 |
| Issue | 11 | Pages | 4308-13 |
| PubMed ID | 15705791 | Mgi Jnum | J:98967 |
| Mgi Id | MGI:3580920 | Doi | 10.1182/blood-2004-09-3578 |
| Citation | Abonia JP, et al. (2005) Constitutive homing of mast cell progenitors to the intestine depends on autologous expression of the chemokine receptor CXCR2. Blood 105(11):4308-13 |
| abstractText | Homing of mast cell progenitors (MCps) to the mouse small intestine involves the interaction of alpha4beta7 integrin with mucosal addressin cellular adhesion molecule-1 (MAdCAM-1). We now demonstrate the dependence of this process on CXC chemokine receptor 2 (CXCR2) and vascular cell adhesion molecule-1 (VCAM-1) using null strains and mice sublethally irradiated and bone marrow (BM) reconstituted (SIBR) with wild-type or null BM or with wild-type BM followed by administration of blocking antibody. The intestinal MCp concentration in CXCR2(-/-) mice was reduced by 67%, but was unaltered in CC chemokine receptor 2(-/-) (CCR2(-/-)), CCR3(-/-), or CCR5(-/-) mice. SIBR mice given CXCR2(-/-) BM had an intestinal MCp concentration that was 76% less than that in BALB/c BM reconstituted mice. Antibody blockade of VCAM-1 or of CXCR2 in SIBR mice reduced intestinal MCp reconstitution, and mice lacking endothelial VCAM-1 also had a marked reduction relative to wild-type mice. Finally, the half-life of intestinal MCps in wild-type mice was less than one week on the basis of a more than 50% reduction by administration of anti-alpha4beta7 integrin or anti-CXCR2. Thus, the establishment and maintenance of MCps in the small intestine is a dynamic process that requires expression of the alpha4beta7 integrin and the alpha-chemokine receptor CXCR2. |
