| First Author | Mardinly AR | Year | 2018 |
| Journal | Nat Neurosci | Volume | 21 |
| Issue | 6 | Pages | 881-893 |
| PubMed ID | 29713079 | Mgi Jnum | J:264713 |
| Mgi Id | MGI:6196794 | Doi | 10.1038/s41593-018-0139-8 |
| Citation | Mardinly AR, et al. (2018) Precise multimodal optical control of neural ensemble activity. Nat Neurosci 21(6):881-893 |
| abstractText | Understanding brain function requires technologies that can control the activity of large populations of neurons with high fidelity in space and time. We developed a multiphoton holographic approach to activate or suppress the activity of ensembles of cortical neurons with cellular resolution and sub-millisecond precision. Since existing opsins were inadequate, we engineered new soma-targeted (ST) optogenetic tools, ST-ChroME and IRES-ST-eGtACR1, optimized for multiphoton activation and suppression. Employing a three-dimensional all-optical read-write interface, we demonstrate the ability to simultaneously photostimulate up to 50 neurons distributed in three dimensions in a 550 x 550 x 100-microm(3) volume of brain tissue. This approach allows the synthesis and editing of complex neural activity patterns needed to gain insight into the principles of neural codes. |
