| First Author | Hanks M | Year | 1995 |
| Journal | Science | Volume | 269 |
| Issue | 5224 | Pages | 679-82 |
| PubMed ID | 7624797 | Mgi Jnum | J:27767 |
| Mgi Id | MGI:75252 | Doi | 10.1126/science.7624797 |
| Citation | Hanks M, et al. (1995) Rescue of the En-1 mutant phenotype by replacement of En-1 with En-2. Science 269(5224):679-82 |
| abstractText | The related mouse Engrailed genes En-1 and En-2 are expressed from the one- and approximately five-somite stages, respectively, in a similar presumptive mid-hindbrain domain. However, mutations in En-1 and En-2 produce different phenotypes. En-1 mutant mice die at birth with a large mid-hindbrain deletion, whereas En-2 mutants are viable, with cerebellar defects. To determine whether these contrasting phenotypes reflect differences in temporal expression or biochemical activity of the En proteins, En-1 coding sequences were replaced with En-2 sequences by gene targeting. This rescued all En-1 mutant defects, demonstrating that the difference between En-1 and En-2 stems from their divergent expression patterns. |
