|  Help  |  About  |  Contact Us

Publication : Vegf regulates embryonic erythroid development through Gata1 modulation.

First Author  Drogat B Year  2010
Journal  Blood Volume  116
Issue  12 Pages  2141-51
PubMed ID  20554972 Mgi Jnum  J:164506
Mgi Id  MGI:4834068 Doi  10.1182/blood-2010-01-264143
Citation  Drogat B, et al. (2010) Vegf regulates embryonic erythroid development through Gata1 modulation. Blood 116(12):2141-51
abstractText  To determine the role of vascular endothelial growth factor (Vegf) in embryonic erythroid development we have deleted or overexpressed Vegf specifically in the erythroid lineage using the EpoR-iCre transgenic line in combination with Cre/loxP conditional gain and loss of function Vegf alleles. ROSA26 promoter-based expression of the Vegf(164) isoform in the early erythroid lineage resulted in a differentiation block of primitive erythroid progenitor (EryP) development and a partial block in definitive erythropoiesis between the erythroid burst-forming unit and erythroid colony-forming unit stages. Decreased mRNA expression levels of the key erythroid transcription factor Gata1 were causally linked to this phenotype. Conditional deletion of Vegf within the erythroid lineage was associated with increased Gata1 levels and increased erythroid differentiation. Expression of a ROSA26-based GATA2 transgene rescued Gata1 mRNA levels and target genes and restored erythroid differentiation in our Vegf gain of function model. These results demonstrate that Vegf modulates Gata1 expression levels in vivo and provides new molecular insight into Vegf's ability to modulate erythropoiesis.
Quick Links:
 
Quick Links:
 

Expression

Publication --> Expression annotations

 

Other

37 Bio Entities

Trail: Publication

0 Expression