| First Author | Layman E | Year | 2022 |
| Journal | STAR Protoc | Volume | 3 |
| Issue | 4 | Pages | 101881 |
| PubMed ID | 36595893 | Mgi Jnum | J:355118 |
| Mgi Id | MGI:7737796 | Doi | 10.1016/j.xpro.2022.101881 |
| Citation | Layman E, et al. (2022) Protocol for assessing phagocytosis activity in cultured primary murine microglia. STAR Protoc 3(4):101881 |
| abstractText | In this protocol, we describe steps to assess inflammation-induced cell response in cultured primary murine microglia through the analysis of fluorescent bead phagocytosis. We detail primary murine mixed glial cell culture preparation followed by microglia-specific isolation. Further, we describe treatment with lipopolysaccharide (LPS) to induce phagocytosis of fluorescent beads, followed by quantitative analysis using fluorescent imaging and Fiji - ImageJ software. For complete details on the use and execution of this protocol, please refer to Parrott et al.(1). |
