| First Author | Jung J | Year | 2023 |
| Journal | Neuron | Volume | 111 |
| Issue | 5 | Pages | 650-668.e4 |
| PubMed ID | 36584679 | Mgi Jnum | J:336664 |
| Mgi Id | MGI:7441832 | Doi | 10.1016/j.neuron.2022.12.015 |
| Citation | Jung J, et al. (2023) mRNA transport, translation, and decay in adult mammalian central nervous system axons. Neuron 111(5):650-668.e4 |
| abstractText | Localized mRNA translation regulates synapse function and axon maintenance, but how compartment-specific mRNA repertoires are regulated is largely unknown. We developed an axonal transcriptome capture method that allows deep sequencing of metabolically labeled mRNAs from retinal ganglion cell axon terminals in mouse. Comparing axonal-to-somal transcriptomes and axonal translatome-to-transcriptome enables genome-wide visualization of mRNA transport and translation and unveils potential regulators tuned to each process. FMRP and TDP-43 stand out as key regulators of transport, and experiments in Fmr1 knockout mice validate FMRP's role in the axonal transportation of synapse-related mRNAs. Pulse-and-chase experiments enable genome-wide assessment of mRNA stability in axons and reveal a strong coupling between mRNA translation and decay. Measuring the absolute mRNA abundance per axon terminal shows that the adult axonal transcriptome is stably maintained by persistent transport. Our datasets provide a rich resource for unique insights into RNA-based mechanisms in maintaining presynaptic structure and function in vivo. |
