| First Author | Vig PJ | Year | 1995 |
| Journal | Res Commun Mol Pathol Pharmacol | Volume | 89 |
| Issue | 3 | Pages | 307-16 |
| PubMed ID | 8680799 | Mgi Jnum | J:29595 |
| Mgi Id | MGI:77122 | Citation | Vig PJ, et al. (1995) Developmental changes in cerebellar endothelin-1 receptors in the neurologic mouse lurcher mutant. Res Commun Mol Pathol Pharmacol 89(3):307-16 |
| abstractText | In order to elucidate the role of neuromodulator endothelin-1 (ET-1) in cerebellar degeneration we determined ET-1 receptor binding in the control and lurcher mice cerebella at different postnatal (P) ages. The lurcher mutant is a suggested animal model for human cerebellar ataxias. The cerebellar membranes were prepared from 5 to 20 day old lurcher mice and littermate controls. ET-1 receptor binding was determined using radioligand assay and affinity cross-linking. There was a significant decrease in [125I]ET-1 receptor binding with increasing postnatal age in the control and lurcher mice cerebella. Lurcher mice showed a significant increase in [125I]ET-1 receptor binding at P 9 to P 20 as compared with the controls. Whereas, at P5 to P7 no significant change was observed. By autoradiography, ET-1 receptors were localized in tile granule cell layer of 15 day old control and lurcher mice cerebella. Whereas, the lUrcher mice showed an increase in [125I]ET-1 binding in the cerebellum as compared with the littermate controls. The results of the present study suggest that the increase in ET-1 receptor binding as early as P9 (when neuronal loss is minimal) in the lurcher mutant may reflect upregulation of ET-1 receptors in the degenerating neurons. At P 15 to P 20, when about 50% to 75% neurons are lost respectively, these changes may also reflect astrogliosis. |
