| First Author | Chen J | Year | 2015 |
| Journal | Blood | Volume | 126 |
| Issue | 24 | Pages | 2621-31 |
| PubMed ID | 26491068 | Mgi Jnum | J:230069 |
| Mgi Id | MGI:5755343 | Doi | 10.1182/blood-2015-06-652453 |
| Citation | Chen J, et al. (2015) IFN-gamma-mediated hematopoietic cell destruction in murine models of immune-mediated bone marrow failure. Blood 126(24):2621-31 |
| abstractText | Interferon gamma (IFN-gamma) has been reported to have both negative and positive activity on hematopoietic cells, adding complexity to the interpretation of its pleiotropic functions. We examined the effects of IFN-gamma on murine hematopoietic stem cells (HSCs) and progenitors in vitro and in vivo by using mouse models. IFN-gamma treatment expanded bone marrow (BM) c-Kit(+)Sca1(+)Lin(-) (KSL) cell number but reduced BM KLCD150(+) and KLCD150(+)CD48(-) cells. IFN-gamma-expanded KSL cells engrafted poorly when tested by competitive repopulation in vivo. KSL, KLCD150(+), and KLCD150(+)CD48(-) cells from IFN-gamma-treated animals all showed significant upregulation in Fas expression. When cocultured with activated T cells in vitro, KSL and KLCD150(+) cells from IFN-gamma-treated donors showed increased apoptosis relative to those from untreated animals, and infusion of activated CD8 T cells into IFN-gamma-injected animals in vivo led to partial elimination of KSL cells. Exposure of BM cells or KSL cells to IFN-gamma increased expression of Fas, caspases, and related proapoptotic genes and decreased expression of Ets-1 and other hematopoietic genes. In mouse models of BM failure, mice genetically deficient in IFN-gamma receptor expression showed attenuation of immune-mediated marrow destruction, whereas effector lymphocytes from IFN-gamma-deficient donors were much less potent in initiating BM damage. We conclude that the activity of IFN-gamma on murine hematopoiesis is context dependent. IFN-gamma-augmented apoptotic gene expression facilitates destruction of HSCs and progenitors in the presence of activated cytotoxic T cells, as occurs in human BM failure. |
