| First Author | Cathcart HM | Year | 2011 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 52 |
| Issue | 7 | Pages | 3984-93 |
| PubMed ID | 21345992 | Mgi Jnum | J:181441 |
| Mgi Id | MGI:5311314 | Doi | 10.1167/iovs.10-6449 |
| Citation | Cathcart HM, et al. (2011) Interferon-gamma, macrophages, and virus spread after HSV-1 injection. Invest Ophthalmol Vis Sci 52(7):3984-93 |
| abstractText | PURPOSE: After uniocular anterior chamber (AC) injection of HSV-1, the anterior segment of BALB/c mice becomes inflamed and infected; however, virus does not spread from the anterior segment to cause retinitis in the injected eye. The purpose of these studies was to determine whether interferon (IFN-)-gamma and Mac-1(+) cells play a role in preventing direct anterior-to-posterior spread of HSV-1 in the injected eye. METHODS: One AC of adult female BALB/c mice was injected with HSV-1 (KOS). The location of IFN-alpha, IFN-beta, and IFN-gamma in the injected eye was determined by immunofluorescence, and mRNA expression was quantified by qPCR. Injected eyes of IFN-gamma knockout or clodronate-treated macrophage-depleted mice were examined to determine whether the absence of IFN-gamma or Mac-1(+) macrophages affected the sites or timing of virus spread. RESULTS: IFN-alpha, IFN-beta, and IFN-gamma were observed in the anterior segment of injected eyes through 72 hours and mRNA levels of IFN-beta and IFN-gamma were increased in virus-infected eyes 48 to 120 hours after infection. However, the absence of IFN-gamma or macrophages did not affect either the sites or the timing of HSV-1 infection in injected eyes. CONCLUSIONS: Protection of the retina of the injected eye does not depend on a single cell type or cytokine. In addition, in the eye, as in other sites of the body, there are redundancies in the innate response to virus infection. |
