| First Author | Sabino GJ | Year | 2011 |
| Journal | Am J Pathol | Volume | 179 |
| Issue | 4 | Pages | 1917-28 |
| PubMed ID | 21820995 | Mgi Jnum | J:176303 |
| Mgi Id | MGI:5290022 | Doi | 10.1016/j.ajpath.2011.06.029 |
| Citation | Sabino GJ, et al. (2011) Interferon-gamma Influences the Composition of Leukocytic Infiltrates in Murine Lyme Carditis. Am J Pathol 179(4):1917-28 |
| abstractText | Interferon (IFN)-gamma is present in lesions of patients with Lyme disease and positively correlates with the severity of manifestations. To investigate the role of IFNgamma in the development of Lyme carditis, wild-type and IFNgamma-deficient C57BL/6 mice were infected with the causative bacterium, Borrelia burgdorferi. Histological analysis revealed no change in the severity of carditis between wild-type and IFNgamma-deficient mice at 14, 21, 25, and 28 days after infection. However, a distinct shift in the types of leukocytes within the hearts of IFNgamma-deficient mice was observed at 25 days. In the absence of IFNgamma, the number of neutrophils in the heart was increased, whereas the number of T lymphocytes was decreased. Bacterial loads within hearts were the same as in wild-type mice. Macrophages secrete chemokines that recruit immune cells, which could contribute to the accumulation of leukocytes in murine Lyme carditis. The ability of IFNgamma and B. burgdorferi to activate murine macrophages was examined, and the two stimuli synergistically induced chemoattractants for mononuclear cells (ie, CXCL9, CXCL10, CXCL11, CXCL16, and CCL12) and decreased those for neutrophils (ie, CXCL1, CXCL2, and CXCL3). IFNgamma and B. burgdorferi also synergistically enhanced secretion of CXCL9 and CXCL10 by murine cardiac endothelial cells. These results indicate that IFNgamma influences the composition of inflammatory infiltrates in Lyme carditis by promoting the accumulation of leukocytes associated with chronic inflammation and suppressing that of cells that typify acute inflammation. |
