| First Author | Yeh CH | Year | 2022 |
| Journal | Immunity | Volume | 55 |
| Issue | 2 | Pages | 272-289.e7 |
| PubMed ID | 35081372 | Mgi Jnum | J:335796 |
| Mgi Id | MGI:6874630 | Doi | 10.1016/j.immuni.2021.12.015 |
| Citation | Yeh CH, et al. (2022) Primary germinal center-resident T follicular helper cells are a physiologically distinct subset of CXCR5(hi)PD-1(hi) T follicular helper cells. Immunity 55(2):272-289.e7 |
| abstractText | T follicular helper (Tfh) cells are defined by a Bcl6(+)CXCR5(hi)PD-1(hi) phenotype, but only a minor fraction of these reside in germinal centers (GCs). Here, we examined whether GC-resident and -nonresident Tfh cells share a common physiology and function. Fluorescently labeled, GC-resident Tfh cells in different mouse models were distinguished by low expression of CD90. CD90(neg/lo) GCTfh cells required antigen-specific, MHCII(+) B cells to develop and stopped proliferating soon after differentiation. In contrast, nonresident, CD90(hi) Tfh (GCTfh-like) cells developed normally in the absence of MHCII(+) B cells and proliferated continuously during primary responses. The TCR repertoires of both Tfh subsets overlapped initially but later diverged in association with dendritic cell-dependent proliferation of CD90(hi) GCTfh-like cells, suggestive of TCR-dependency seen also in TCR-transgenic adoptive transfer experiments. Furthermore, the transcriptomes of CD90(neg/lo) and CD90(hi) GCTfh-like cells were enriched in different functional pathways. Thus, GC-resident and nonresident Tfh cells have distinct developmental requirements and activities, implying distinct functions. |
