| First Author | Crowley MT | Year | 1999 |
| Journal | J Immunol | Volume | 163 |
| Issue | 9 | Pages | 4894-900 |
| PubMed ID | 10528191 | Mgi Jnum | J:118436 |
| Mgi Id | MGI:3699574 | Doi | 10.4049/jimmunol.163.9.4894 |
| Citation | Crowley MT, et al. (1999) Influence of lymphocytes on the presence and organization of dendritic cell subsets in the spleen. J Immunol 163(9):4894-900 |
| abstractText | Studies were undertaken to clarify the roles of individual leukocyte populations in maintaining the presence and organization of splenic dendritic cells (DCs). Using Abs specific for DC subsets, we found that the distinct types of DC maintained appropriate compartmentalization within the white pulp of lymphocyte-deficient mice despite an unusual overall distribution of DCs. Even in mice lacking both B and T lymphocytes, the central arteriole remained the structure around which T area DCs were organized. Marginal zone area DCs remained in a peripheral sheath excluded from the T area DCs. Additionally, we revealed an important role for splenic B cells in the presence and organization of marginal zone cells. B-deficient or B- and T-deficient mice lacked sialoadhesin+ marginal zone macrophages and lacked MAdCAM-1 expression in marginal zone reticular endothelial cells. Adoptive transfer of B lymphocytes induced MAdCAM-1 expression but failed to recruit marginal zone macrophages. Taken together, our results demonstrate that the arrival, localization, and persistence of DCs in spleen are events not solely dependent upon signals from the mature B and T cells or marginal zone macrophages. We suggest that specific stromal elements in the vicinity of the central arteriole are primarily responsible for providing directional cues to the DC. |
