| First Author | Meher AK | Year | 2014 |
| Journal | Am J Pathol | Volume | 184 |
| Issue | 11 | Pages | 3130-41 |
| PubMed ID | 25194661 | Mgi Jnum | J:216374 |
| Mgi Id | MGI:5608728 | Doi | 10.1016/j.ajpath.2014.07.006 |
| Citation | Meher AK, et al. (2014) B2 cells suppress experimental abdominal aortic aneurysms. Am J Pathol 184(11):3130-41 |
| abstractText | Recent reports of rupture in patients with abdominal aortic aneurysm (AAA) receiving B-cell depletion therapy highlight the importance of understanding the role of B cells (B1 and B2 subsets) in the development of AAA. We hypothesized that B2 cells aggravate experimental aneurysm formation. The IHC staining revealed infiltration of B cells in the aorta of wild-type (C57BL/6) mice at day 7 after elastase perfusion and persisted through day 21. Quantification of immune cell types using flow cytometry at day 14 showed significantly greater infiltration of mononuclear cells, including B cells (B2: 93% of total B cells) and T cells in elastase-perfused aortas compared with saline-perfused or normal aortas. muMT (mature B-cell deficient) mice were prone to AAA formation similar to wild-type mice in two different experimental AAA models. Contradicting our hypothesis, adoptive transfer of B2 cells suppressed AAA formation (102.0% +/- 7.3% versus 75.2% +/- 5.5%; P < 0.05) with concomitant increase in the splenic regulatory T cell (0.24% +/- 0.03% versus 0.92% +/- 0.23%; P < 0.05) and decrease in aortic infiltration of mononuclear cells. Our data suggest that B2 cells constitute the largest population of B cells in experimental AAA. Furthermore, B2 cells, in the absence of other B-cell subsets, increase splenic regulatory T-cell population and suppress AAA formation. |
