| First Author | Alsén S | Year | 2022 |
| Journal | Front Immunol | Volume | 13 |
| Pages | 813203 | PubMed ID | 35355990 |
| Mgi Jnum | J:322859 | Mgi Id | MGI:7259009 |
| Doi | 10.3389/fimmu.2022.813203 | Citation | Alsen S, et al. (2022) Antigen-Presenting B Cells Program the Efferent Lymph T Helper Cell Response. Front Immunol 13:813203 |
| abstractText | B cells interact with T follicular helper (Tfh) cells in germinal centers (GCs) to generate high-affinity antibodies. Much less is known about how cognate T-B-cell interactions influence Th cells that enter circulation and peripheral tissues. Therefore, we generated mice lacking MHC-II expressing B cells and, by thoracic duct cannulation, analyzed Th cells in the efferent lymph at defined intervals post-immunization. Focusing on gut-draining mesenteric lymph nodes (MLNs), we show that antigen-specific alpha4beta7 (+) gut-homing effector Th cells enter the circulation prior to CXCR5(+)PD-1(+) Tfh-like cells. B cells appear to have no or limited impact on the early generation and egress of gut-homing Th cells but are critical for the subsequent appearance of Tfh-like cells that peak in the lymph before GCs have developed. At this stage, antigen-presenting B cells also reduce the proportion of alpha4beta7 (+) Th cells in the MLN and efferent lymph. Furthermore, cognate B-cell interaction drives a broad transcriptional program in Th cells, including IL-4 that is confined to the Tfh cell lineage. The IL-4-producing Tfh-like cells originate from Bcl6(+) precursors in the LNs and have gut-homing capacity. Hence, B cells program the efferent lymph Th cell response within a limited window of time after antigenic challenge. |
