| First Author | Haque SF | Year | 2013 |
| Journal | Biochem Biophys Res Commun | Volume | 441 |
| Issue | 2 | Pages | 482-7 |
| PubMed ID | 24383080 | Mgi Jnum | J:320616 |
| Mgi Id | MGI:6875055 | Doi | 10.1016/j.bbrc.2013.10.087 |
| Citation | Haque SF, et al. (2013) The Elambda(3-1) enhancer is essential for V(D)J recombination of the murine immunoglobulin lambda light chain locus. Biochem Biophys Res Commun 441(2):482-7 |
| abstractText | Enhancers are essential for long range chromatin opening and the activation of V(D)J recombination at the antigen receptor loci. The murine immunoglobulin lambda light chain locus is a duplicated locus and, using a bacterial artificial chromosome spanning the 3' half of the locus to generate transgenic mice, we have identified a critical enhancer element for lambda locus recombination. Four hypersensitive sites had been previously mapped downstream of the JClambda1 gene segment (HS1-4). Systematic deletion of these individual hypersensitive sites showed that HS1, which forms the major part of the transcription enhancer, Elambda3-1, is essential for Iglambda recombination and that it also helps to restrict Iglambda stage-specific recombination. |
