| First Author | Beyersdorf N | Year | 2007 |
| Journal | Eur J Immunol | Volume | 37 |
| Issue | 12 | Pages | 3445-54 |
| PubMed ID | 18034419 | Mgi Jnum | J:128529 |
| Mgi Id | MGI:3767381 | Doi | 10.1002/eji.200737126 |
| Citation | Beyersdorf N, et al. (2007) Characterization of mouse CD4 T cell subsets defined by expression of KLRG1. Eur J Immunol 37(12):3445-54 |
| abstractText | The mouse killer cell lectin-like receptor G1 (KLRG1) is an inhibitory receptor known to be expressed on a subset of NK cells and antigen-experienced CD8 T cells. Here, we have characterized expression of KLRG1 on CD4(+) T cells from normal mice. While a polyclonal TCR repertoire suggests thymic origin of KLRG1(+) CD4(+) cells, KLRG1 expression was found to be restricted to peripheral CD4(+) T cells. Based on phenotypic analyses, a minority of KLRG1(+) CD4(+) cells are effector/memory cells with a proliferative history. The majority of KLRG1(+) CD4(+) cells are, however, bona fide Treg cells that depend on IL-2 and/or CD28 and express both FoxP3 and high levels of intracellular CD152. KLRG1-expressing Treg are contained within the CD38(+) subset but are only partially overlapping with the CD25(+) CD4(+) Treg subset. In functional assays, KLRG1(+) CD4(+) cells were anergic to TCR stimulation with respect to proliferation, and sorted KLRG1(+) CD25(+) CD4(+) cells were equal or superior to KLRG1(+) CD25(-) CD4(+) cells, which were more potent than KLRG1(-) CD25(+) CD4(+) cells in suppressing responder cell proliferation. Together, our results demonstrate that KLRG1 expression defines novel and distinctive subsets of senescent effector/memory and potent regulatory CD4(+) T cells. |
