| First Author | Rosenbaum JT | Year | 1998 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 39 |
| Issue | 1 | Pages | 64-9 |
| PubMed ID | 9430546 | Mgi Jnum | J:45408 |
| Mgi Id | MGI:1195393 | Citation | Rosenbaum JT, et al. (1998) Interleukin-6 does not mediate endotoxin-induced uveitis in mice: studies in gene deletion animals. Invest Ophthalmol Vis Sci 39(1):64-9 |
| abstractText | PURPOSE. Interleukin-6 (IL-6) has been strongly implicated in anterior uveitis based on its presence in aqueous humor from infected eyes and its inflammatory effects when injected intravitreally into rats. We used IL-6-deficient mice to test further the hypothesis that nd contributes to the development of endotoxin-induced uveitis. METHODS. Uveitis was scored by histologic analysis of C3H/ HeN mice 24 hours after intravitreal injections of up to 200 ng of recombinant murine IL-6. Uveitis was similarly measured in IL-6 deficient mice and congenic controls 24 hours after intravitreal injection of 250 ng of Escherichia coli endotoxin. Reverse transcription- polymerase chain reaction was used to detect mRNAs for several cytokines at 3 hours postinjection. The IL-6 concentration in aqueous humor samples was determined with a bioassay using the murine B9 plasmacytoma cell line. RESULTS. Direct injection of IL-6 did not induce uveitis. Mice genetically deficient in IL-6 developed endotoxin- induced uveitis that was comparable or more severe than congenic control mice. Compensatory changes in the expression of mRNA for other cytokines were not detected in irises from the IL-6-deficient mice. In IL-6-competent mice that received bilateral endotoxin injections, no correlation was found between the number of infiltrating cells in one eye and the IL-6 concentration in the aqueous humor of the contralateral eye. CONCLUSIONS. In marked contrast to previous conclusions with rats, IL-6 was not sufficient for inducing uveitis in mice. Additionally, IL-6 was not necessary for the development of uveitis subsequent to intravitreal injection of endotoxin in mice. |
