| First Author | Prato CA | Year | 2020 |
| Journal | iScience | Volume | 23 |
| Issue | 7 | Pages | 101278 |
| PubMed ID | 32619699 | Mgi Jnum | J:349850 |
| Mgi Id | MGI:6717688 | Doi | 10.1016/j.isci.2020.101278 |
| Citation | Prato CA, et al. (2020) Galectin-8 Enhances T cell Response by Promotion of Antigen Internalization and Processing. iScience 23(7):101278 |
| abstractText | Galectin-8 (Gal-8) is a mammalian lectin endowed with immunostimulatory ability. In the present work, we demonstrate that Gal-8-glycan interactions on the surface of antigen-presenting cells (APCs) promote antigen binding and internalization, independently from antigen nature. Both Gal-8 and antigen were together internalized and localized in early endosomes. Interestingly, antigen processing by APCs was also accelerated in the presence of Gal-8 as a separate mechanism, distinct from the increased antigen internalization. Moreover, APCs pulsed together with antigen and Gal-8 were able to activate cognate CD4(+) T cells more efficiently than those pulsed with antigen alone. This enhanced antigen presentation was still evident in the absence of costimulatory signals and APCs-derived soluble mediators. Therefore, our results provide evidence for as yet unrecognized mechanism by which Gal-8 stimulates the elicitation of the immune response in a lectin-dependent manner, by inducing antigen uptake and processing upon lattice formation at APCs surface. |
