| First Author | Pawlik TM | Year | 2003 |
| Journal | Int J Mol Med | Volume | 12 |
| Issue | 4 | Pages | 541-8 |
| PubMed ID | 12964032 | Mgi Jnum | J:113636 |
| Mgi Id | MGI:3687115 | Citation | Pawlik TM, et al. (2003) Central role of interleukin-6 in burn induced stimulation of hepatic amino acid transport. Int J Mol Med 12(4):541-8 |
| abstractText | Burn injury produces a marked and prolonged hypermetabolic state, which is characterized by accelerated hepatic amino acid metabolism and negative nitrogen balance. The effect of burn injury on hepatocyte transport of glutamine, a key substrate in gluconeogenesis and ureagenesis, was examined following the administration of a full thickness 20% total body surface scald injury. The burn injury was induced in wild-type mice, as well as two types of knockout mice: a CD-14 knockout and an interleukin-6 (IL-6) knockout. In both the wild-type and CD-14 knockout mice a latent and profound 2-fold increase in hepatocyte glutamine transport was seen. In contrast, hepatocytes isolated from IL-6 knockout mice failed to show an induction of glutamine transport after burn injury. Hepatocytes isolated from burned wild-type and CD-14 knockout mice produced significantly higher levels of IL-6 as compared to hepatocytes isolated from sham burned mice. Histologic analysis of skin isolated from wild-type mice showed a robust inflammatory response whereas skin from IL-6 deficient mice showed minimal inflammation and decreased granulation tissue. The results of this study suggest that IL-6 may play a key role in the stimulation of hepatic glutamine transport following burn injury. |
