Primary Identifier | MGI:3626301 | Allele Type | Targeted |
Attribute String | Inserted expressed sequence | Gene | Igh-J |
Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
Is Recombinase | false | Is Wild Type | false |
description | Targeting took place in ES cells that contain the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. |
molecularNote | A targeting vector containing the mAb 6.121 VHDHJH sequence and a loxP-flanked neomycin resistance cassette was inserted into the locus to replace the JH region. Cre mediated recombination subsequently removed the neomycin cassette upon transmission from male chimeric mice. |