| First Author | Levelt CN | Year | 1995 |
| Journal | Immunity | Volume | 3 |
| Issue | 2 | Pages | 215-22 |
| PubMed ID | 7648394 | Mgi Jnum | J:28295 |
| Mgi Id | MGI:75916 | Doi | 10.1016/1074-7613(95)90091-8 |
| Citation | Levelt CN, et al. (1995) Regulation of thymocyte development through CD3: functional dissociation between p56lck and CD3 sigma in early thymic selection. Immunity 3(2):215-22 |
| abstractText | We studied the extent of functional linkage between CD3 sigma and p56lck in pre-TCR-dependent thymocyte development. Differentiation of DN to DP cells was examined by treatment of RAG2/CD3 sigma and RAG1/p56lck double-deficient mice with anti-CD3 epsilon antibodies. The results suggest that CD3 sigma has no specific role in this maturation step, but may be important for amplification of signaling through the pre-TCR. In contrast, p56lck is the main protein tyrosine kinase associated with signaling through the pre-TCR-CD3 complex. In DP thymocytes, the Ca2+ response to anti-CD3 epsilon was totally abolished in CD3 sigma-I-but only reduced in p56lck-I-mice, and in vivo responses to anti-CD3 epsilon differed from one another. Thus, CD3 sigma and p56lck are functionally not tightly associated and their deficiencies cause distinct developmental defects. |
