| First Author | Picataggi A | Year | 2013 |
| Journal | Mamm Genome | Volume | 24 |
| Issue | 7-8 | Pages | 257-65 |
| PubMed ID | 23722970 | Mgi Jnum | J:199101 |
| Mgi Id | MGI:5500847 | Doi | 10.1007/s00335-013-9459-x |
| Citation | Picataggi A, et al. (2013) A coding variant in SR-BI (I179N) significantly increases atherosclerosis in mice. Mamm Genome 24(7-8):257-65 |
| abstractText | Human coding variants in scavenger receptor class B member 1 (SR-BI; gene name SCARB1) have recently been identified as being associated with plasma levels of HDL cholesterol. However, a link between coding variants and atherosclerosis has not yet been established. In this study we set out to examine the impact of a SR-BI coding variant in vivo. A mouse model with a coding variant in SR-BI (I179N), identified through a mutagenesis screen, was crossed with Ldlr (-/-) mice, and these mice were maintained on a Western-type diet to promote atherosclerosis. Mice showed 56 and 125 % increased atherosclerosis in female and male Ldlr (-/-) Scarb1 (I179N) mice, respectively, when compared to gender-matched Ldlr (-/-) control mice. As expected, HDL cholesteryl ester uptake was impaired in Ldlr (-/-) Scarb1 (I179N) mice compared to Ldlr (-/-) control mice, with a net effect of increased small and very small LDL cholesterol in Ldlr (-/-) Scarb1 (I179N) mice being the most probable cause of the observed increased atherosclerosis. Our data show that non-null coding variants in SR-BI can have a large significant impact on atherosclerosis, even if plasma lipid levels are not dramatically affected, and that human mutations in other candidate lipid genes could significantly impact atherosclerosis. |
