| First Author | Saxena V | Year | 2022 |
| Journal | Cell Rep | Volume | 39 |
| Issue | 3 | Pages | 110727 |
| PubMed ID | 35443187 | Mgi Jnum | J:336506 |
| Mgi Id | MGI:7283946 | Doi | 10.1016/j.celrep.2022.110727 |
| Citation | Saxena V, et al. (2022) Treg tissue stability depends on lymphotoxin beta-receptor- and adenosine-receptor-driven lymphatic endothelial cell responses. Cell Rep 39(3):110727 |
| abstractText | Regulatory T cell (Treg) lymphatic migration is required for resolving inflammation and prolonging allograft survival. Focusing on Treg interactions with lymphatic endothelial cells (LECs), we dissect mechanisms and functional consequences of Treg transendothelial migration (TEM). Using three genetic mouse models of pancreatic islet transplantation, we show that Treg lymphotoxin (LT) alphabeta and LEC LTbeta receptor (LTbetaR) signaling are required for efficient Treg migration and suppressive function to prolong allograft survival. Inhibition of LT signaling increases Treg conversion to Foxp3(lo)CD25(lo) exTregs. In a transwell-based model of TEM across polarized LECs, non-migrated Tregs become exTregs. Such conversion is regulated by LTbetaR nuclear factor kappaB (NF-kappaB) signaling in LECs, which increases interleukin-6 (IL-6) production and drives exTreg conversion. Migrating Tregs are ectonucleotidase CD39(hi) and resist exTreg conversion in an adenosine-receptor-2A-dependent fashion. Human Tregs migrating across human LECs behave similarly. These molecular interactions can be targeted for therapeutic manipulation of immunity and suppression. |
